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What kind of CRISPR library are you designing?

What kind of guide RNA scoring algorithm do you want us to build?

What is your data source for building this algorithm?

What nuclease are we building this scoring algorithm for?

Select your knockin experiment. Learn more about CRISPR knockin:

Select your nuclease(s).

What reference genome are you using?

Which cell line(s) are you using?

e.g. HEK293T, K562. Skip this question if you are unsure. Learn more about model characterization:
How many targets do you have?

Skip if unsure.
Securely upload your gene or target list.

Upload in .doc, .csv or other format. Skip this step for now if you haven’t finalized your list.
What kind of selection method / reporter system are you using?

Describe your strategy of selecting cells containing sgRNAs (e.g. GFP, puromycin, anti-CRISPR-Cas9 antibodies). Skip this question if unsure.
Select your guide delivery method.

Skip this question if unsure. Learn more about CRISPR delivery methods:

Select your vector system.

How will you screen?

How will you screen?

Almost done! Are there any additional features you wish to add to your library?

Describe your custom parameters below (e.g. GC content, custom PAM site, protospacer length). Learn more about key experimental considerations:
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We are excited to help design your CRISPR experiment.
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